IVT mRNA Purification Services
There has been tremendous interest around in vitro-transcribed (IVT) mRNA for research and therapeutic applications in recent years. The manufacturing of pure mRNA products are on-demand as the importance of IVT mRNA is gradually rising. With years of experience in IVT mRNA synthesis and purification technology, Creative Biogene has developed robust, and cost-effective protocols to purify IVT mRNA to help worldwide customers obtain efficient and high-quality synthetic mRNAs.
The importance of purification
Schematic of IVT mRNA purification.
The purity of IVT mRNA is a key factor in determining the efficiency of target antigen expression. After in vitro transcription, the reaction solution contains mRNAs and remaining reagents. The remaining reagents of the in vitro synthesis process are comprised of RNA polymerase, unreacted nucleotides, CAP analogues, DNA template as well as double-stranded RNA (dsRNA) side products. Previous studies have identified that the presence of certain byproducts can trigger cellular immune responses. Thus, purifying mRNA from these contaminant impurities is critical, especially for in vivo applications.
Creative Biogene' IVT mRNA purification protocols
Many purification techniques have been developed to enhance the concentration and quality of IVT mRNA, including high-performance liquid chromatography (HPLC), fast protein liquid chromatography (FPLC) and magnetic beads technology.
For large-scale purification and isolation, high performance liquid chromatography (HPLC) is most often used. Previous studies demonstrated that the translation efficiency of HPLC-purified mRNA is greatly enhanced. HPLC can be used to isolate synthesized mRNA from the remaining reagents via size exclusion or ion-exchange columns, thereby removing smaller or larger by-products, such as dsRNA, abortive transcripts and so on.
Fast protein liquid chromatography (FPLC) is modern liquid chromatography with a principle similar to HPLC. FPLC can maintain the fast and high-resolution characteristics of HPLC and has a large column capacity, high recovery efficiency, and can easily activate biological macromolecules. It has been widely used to separate proteins, polypeptides, and oligonucleotides.
Magnetic beads are made up of tiny (20 to 30 nm) particles of iron oxides, which give them superparamagnetic properties. In contrast to prior purification methods, this method is fast, consistently reliable, and the most efficient in mRNA purification. Under optimized conditions, mRNA selectively binds to the surface of magnetic beads, while other contaminants stay in in vitro transcription reaction solution.
Advantages of our IVT mRNA purification service
- High purity and high yield at a competitive price
- Advanced and robust technology platform
- Skillful team of experts
- Best after-sale service
To meet the particular needs of global customers, Creative Biogene provides the high-purity and high-yield IVT mRNA based on advanced and robust technology platforms. We believe that our service will make your project achieve twice the result with half the effort. Please don't hesitate to contact us if you have any demands for IVT mRNA purification.