mRNA Stability Measurement and Analysis
mRNA stability is the major control point in gene expression regulation. Changes in mRNA stability are intimately related to alterations in gene expression regulation. For envisaged medical applications, mRNA stability is a vital parameter because it determines the specific implementation of mRNA therapy, such as the dosing and interval of administration. Creative Biogene is your reliable partner in mRNA research. With years of experience in this area, our experts have developed a well-established platform for mRNA stability analysis.
Strategies for optimizing mRNA stability and translation
To increase the stability and translation of in vitro-transcribed (IVT) mRNA administered to cells or organisms, several strategies have been developed. With year of accumulation in mRNA research, we have grouped these strategies into two categories
Stable translation of IVT mRNA requires a functional 5' cap structure. The structure has an impact on innate sensing and protein production. Synergy with the 5'cap and other determinants, the poly(A) tail regulates the stability and translation efficiency of the IVT mRNA through its length. In addition, other elements of IVT mRNA, including the codon composition of the region (ORF, its dinucleotides composition), and untranslated region (UTR, their sequences, length, and secondary structures), also are important for the regulation of the translation of the mRNA and the protein expression.
mRNA intracellular delivery system
In addition to optimizing an mRNA construct, in vivo mRNA delivery is critical to increased mRNA stability and translation. Because mRNA is a transient molecule by nature and is susceptible to degradation by nuclease activity, thus efficient protection is required. The current delivery systems (such as lipids-based delivery system, polymers-based delivery system, and hybrid system) and the improved injection strategies (including electroporation of mRNA as well as gene gun-based administration) greatly promote the successful delivery of IVT mRNA into cells or organisms.
To date, various approaches have been developed to achieve analysis of mRNA stability and turnover. Most of the measurements present averaged responses and are limited for the computational analysis of the underlying biochemical network. To help customers to acquire additional information about heterogeneity in transfection experiments, we have developed a platform based on the combination of automated time-lapse microscopy and micropatterned arrays, which allows efficient analysis of mRNA stability and translational efficiency at the single-cell level.
We are long-term devoted to the development of mRNA stability analysis. Here we have established a novel platform for mRNA stability estimation and analysis based on the combination analysis of high-throughput sequencing data. Based on our accurate detection data and robust analysis process, our excellent scientists can achieve analysis and characterization mRNA stability genome-wide in good accuracy and sensitivity manner.
Actinomycin D assay is readily applied on a genome-wide level, and is highly cost-effective. With appropriate guidelines and controls, the actinomycin D-based method is a versatile, effective tool for measuring endogenous mRNA stability. We offer a one-stop solution for mRNA stability analysis using actinomycin D assay, including cell cultures, cell treatment with actinomycin D, mRNA quantitation, as well as results analysis.
For any requested information for our mRNA stability measurement and analysis service, please contact us. We look forward to providing services for your next project.