mRNA Stability Analysis Using High-Throughput Sequencing
Modulation of mRNA stability as a powerful tool plays an important role in controlling gene expression during the physiological transitions and regulatory processes. Therefore, a series of approaches for measuring mRNA stability are developed. However, most of the methods are labor-intensive, have limited sensitivity, and/or disrupt normal cellular processes. Creative Biogene is long-term devoted to the development of mRNA stability analysis. Here we have established a novel platform for mRNA stability estimation and analysis based on the combined analysis of high-throughput sequencing data. Based on our accurate detection data and robust analysis process, our service can achieve analysis and characterization mRNA stability with good accuracy and sensitivity.
Novel strategy to characterize mRNA stability
So far, mRNA stability and turnover have been measured by various approaches, such as using actinomycin D, metabolic labeling. The use of actinomycin D is a classical method, which is able to determine the decay in RNA abundance over time following inhibition of transcription. Metabolic labeling is based on metabolic labeling of RNA transcripts with modified nucleotides, allowing less disruption to cellular physiology. Each of these methods has its own advantages. However, many of them have limited sensitivity for low-abundance transcripts and the measurement results vary greatly across assays. Recently, the analysis of high-throughput RNA-seq data is emerging as a promising method for estimation mRNA stability. The novel strategy is based on intronic reads, producing a relative measure of RNA half-life under the assumption of equilibrium conditions between RNA production and decay. Compared with the existing methods based on metabolic labeling or transcriptional inhibition, the approach is efficient, relatively non-disruptive and so on.
Stability analysis of mRNA
Creative Biogene is long-term devoted to the development of mRNA stability analysis. We offer a range of innovative and high-quality services. Based on a team of professionally trained scientists, we are devoted to analyzing mRNA stability based on the combination of high-throughput sequencing data sets. The improved approach is based on two standard and applicable experimental protocols. Our service requires no time course, metabolic labeling, or transcriptional inhibition, allowing effective analysis for mRNA stability undergoing cell-type- or condition-specific decay. The specific services include Precision Run-On and sequencing (PRO-seq) and RNA sequencing (RNA-seq), data preparation and processing, read mapping and transcript abundance estimation, as well as estimation of mRNA stability from these data sets.
Benefits of our service
- Sensitivity for low-abundance transcripts.
- Robust analytical workflow.
- Good applicability, available to various cell types, and can be extended to tissue samples.
Creative Biogene is a well-recognized expert as well as a leading service provider in the field of mRNA analysis. We believe that our service will make your project achieve twice the result with half the effort. If you want to learn more about our mRNA stability analysis service, please don't hesitate to contact us!
- Alkallas, R., et al. (2017). "Inference of RNA decay rate from transcriptional profiling highlights the regulatory programs of Alzheimer's disease." Nature communications, 8(1), 1-11.