mRNA Stability Analysis Using Single-Cell Micro-Arrays

Creative Biogene is your reliable partner in mRNA research. With years of experience in this area, our experts have developed a well-established platform for mRNA stability analysis based on a series of methods. Here, we have developed a platform based on the combination of automated time-lapse microscopy and micropatterned arrays, allowing efficient analysis of mRNA stability and translational efficiency at the single-cell level. The method we offered is based on the high-throughput monitoring of fluorescent reporter protein expression after mRNA transfection, which represents the distribution of individual mRNA expression and degradation rates within a single cell. With the help of our service, customers from all over the world can screen and compare different mRNA constructs in a more rapid and standardized manner.

mRNA analysis at the single-cell level

When developing new mRNA therapeutics, measurement of mRNA functional stability and its expression are two major factors to be considered. At present, a variety of approaches have been developed to achieve analysis of mRNA stability and turnover, including quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), mass spectrometry, metabolic labeling, and fluorescence labeling techniques. Most of the measurements present averaged responses and are limited for the computational analysis of the underlying biochemical network. Because these approaches provide ensemble data that represent the meaning of all cells, reckoning without the fluctuations in the expression amounts as well as the timing of expression. While, micro-array-based single-cell analysis is another means to characterize the properties of mRNA constructs, including the mRNA functional stability as well as its protein expression. mRNA analysis at the single-cell level can help customers to acquire additional information about heterogeneity in transfection experiments.

The microstructured, cell-adhesive substrates for single-cell time-lapse microscopy. (Ferizi, M., et al., 2015)

Stability analysis of IVT mRNA

Based on single-cell microarray technology, we offer an IVT mRNA stability analysis service to satisfy our customers' mRNA functional stability and its expression determination needs. The microstructure single-cell arrays allow us parallel, quantitative measurements of mRNA stability, thereby, our customers can acquire detailed time-resolved data and monitor protein expression dynamics at the single-cell level. Moreover, the regular arrangement of cells not only guarantees reproducible microenvironments but also provides the possibility for fast and automated image analysis. Our platform is also based on automated time-lapse microscopy and automated image analysis. Therefore, we are able to analyze and compare the stability of different mRNA constructs at the single-cell level in a rapid and standardized manner, involving the determination of the functional mRNA half-life as well as the protein half-life and its expression rates.

Benefits of our service

• Professional technical support
• Automated analyzers and workflow
• Fully developed and improved analytical methods
• Data management options

Creative Biogene is a well-recognized expert as well as a leading service provider in the field of mRNA analysis. We believe that our service will make your project achieve twice the result with half the effort. If you want to learn more about our mRNA stability analysis service, please don't hesitate to contact us!