Polymer-Based mRNA Delivery System
Polymers and their derivatives are another commonly used family for mRNA delivery carrier, following lipidic materials. Since polymeric materials are more cost-effective and easier to up-scale the production with their long stability profiles, the polymer-based delivery systems have shown considerable potential in mRNA therapeutics that related to protein replacement, vaccines and other applications. Creative Biogene is a customized service provider in mRNA-based drug research and development (R&D), with a focus on mRNA delivery system development. Equipped with state-of-the-art facility and trained support technicians, we have established a systematic and flexible platform to develop a series of mRNA delivery systems, including polymer-based delivery systems. With years of efforts, we have developed some polymeric or polymers-derived materials and investigated a library of polymer-based mRNA delivery systems. We are confident to help global customers to obtain multiple excellent tissue-targeted mRNA delivery systems, greatly facilitating their project to the next stage. Our careful design, proper selection of the chemistry blocks and control over the targeting properties of the delivery system will secure their safety, viability and versatility.
The polymer-based system for mRNA delivery
Cationic polymers interact with IVT mRNA via charge-charge interaction, leading to the formation of polyplexes which generally present higher stability than lipoplexes. A major advantage of polymer-based delivery system is that possible control their structure, chemical properties and functionalization to adapt to different active substances. With the increasing need of vehicles for mRNA therapy, many different cationic polymers have been synthesized, evaluated and applied for mRNA complexation. The most used polymers include linear or branched polyethyleneimine (PEI), polyacrylates, poly(β-amino esters) (PBAEs) and among others. Among them, PEI was one of the first and widely used polymers for nucleic acid delivery. Since the present of a large number of amine groups in its structure, PEI is conferred a positive charge, thus having a strong affinity to nucleic acids, including mRNA. The positive charge also facilitates the polyplexes to entry into the target cell. In addition, beside the most used polymers, other kinds of polyplexes have been developed for IVT mRNA delivery, such as charge-altering releasable transporters (CARTs), amino polyesters (APEs), and chitosan (a biocompatible cationic glycopolymer containing amines). CARTs are one of representative biodegradable polymers and have demonstrated capacity to deliver mRNA effectively in vivo (including different cell lines and animal models). APEs are synthesized via ring opening polymerization of lactones, having the feature of biodegradable, ionizable, and tissue-selective for mRNA delivery.
Fig1. Polymeric system. (Gómez-Aguado, I., et.al., 2020)
Service offering in Creative Biogene
With year experience in the development of mRNA delivery system, we are able to design and evaluate plenty of polymers for mRNA delivery, and investigate the effects of synthetic parameters of polymers on mRNA delivery performance. The synthetic parameters include polymerization form and degree, molecular structure, hydrophobic core, hydrophilic chain, and among others. In consideration of safety, transfection efficiency as well as intracellular fate, we choose the proper chemistry blocks of these nanocarriers according to customers' specific project goals. The architectures of the individual polymer components can be precisely controlled, and then direct the assembly and transformations into the mature polymer-based delivery system. Moreover, smart components can be incorporated into polymer-based systems to improve their tissue targeting performance, facilitating the passage of biological barriers. Importantly, our service involves crosslinked knedel-like nanoparticles and polymeric micelles, but not limited to them.
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In addition to lipid-based delivery system, polymer-based delivery system has attracted much attention in the area of mRNA therapy. Based on polymerization chemistries and orthogonal chemical modification reactions, the engineering of polymer-based delivery systems can be controlled. Creative Biogene is passionate about strong customer orientation and high-quality service. We continuously challenge ourselves to stay ahead and remain one of the leading mRNA delivery service providers worldwide. In addition, we focus on quality and speed as well as cost, to ensure that the intracellular mRNA delivery is supported in the most effective way. If you are interested in our services, please contact us for more information. We are glad to cooperate with you!
- Guan, S., & Rosenecker, J. (2017). "Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems." Gene therapy, 24(3), 133-143.
- Gómez-Aguado, I., et.al. (2020). "Nanomedicines to deliver mRNA: state of the art and future perspectives." Nanomaterials, 10(2), 364.