Lipid-Polymer Nanoparticles (LPNs)
Fig1. The process of formulating LPNs
through a microfluidic device.
(Zhao, W., et al, 2018)
As a new class of therapeutics, messenger RNA (mRNA) has been extensively explored in versatile biomedical applications, thus triggering an expansion of research into the delivery of in vitro-transcribed (IVT) mRNAs for a variety of therapeutic purposes. To overcome a number of barriers of therapeutic mRNA delivery and extend the application of mRNA therapeutics, considerable effort has been dedicated to the development of delivery vehicles with better performance that can efficiently transport mRNA to target cells in vivo. Consequently, lipid-polymer nanoparticles (LPNs) evolved as a promising class of biomaterials for mRNA delivery, integrating the physicochemical properties of both lipids and polymers. Creative Biogene is a leading service provider in the field of mRNA delivery. Our scientists have years of extensive experience in developing mRNA delivery system. We are dedicated to offering optimal mRNA delivery vehicles for our clients' specific applications.
Efficient delivery of mRNA is one of the key challenges. Suitable delivery systems are urgently needed to increase mRNA uptake and deliver mRNA-based drugs to cells. Most RNA delivery approaches are based on nanoparticles, comprising cationic lipids or polymers. However, due to the needs of specific cell types or a particular therapeutic intervention, tailored vehicles are required and should be further improved. Lipid-polymeric nanoparticles (LPNs) are comprised of both lipids and polymers, representing an approach for the assembly of advanced, next-generation delivery systems. As a new type of nanomaterial for mRNA delivery, LPNs combine the advantageous physical properties of polymers and lipids in the nanoparticles. At present, different LPN production methods have been reported, including the single and multiple step protocols, using nanoprecipitation or solvent evaporation-based manufacturing routes.
At Creative Biogene, we provide different manufacturing protocols (such as changing lip ratios and sequential assembly routes) to assemble hybrid polymer and lipid nanoparticles. The polymer−lipid nanoparticles can be optimized in terms of polymer synthesis and nanoparticle formulation to achieve multiple order-of-magnitude increases in potency. For acquiring better biological activity, the particles are recommended to be designed with a core-shell organization.
Fig2. Graphical representation of the concept of different assembly routes for the preparation of hybrid particles.
For the complex systems of hybrid nanoparticles that are based on the combination of biopolymer, lipid, and mRNA, the control of product quality and the understanding of structure-function coherencies are of fundamental importance. Once hybrid nanoparticles are generated, we also offer physicochemical and biological characterization services and ex vivo experiments to further analyze and verify the particles. We will provide the experiment reports that contain the results of size and zeta potential measurements of the hybrid nanoparticles, as well as the mRNA release results.
Our hybrid polymer and LNP production can be tailored to a complex mRNA delivery system and employed for a given therapeutic intervention, such as vaccine development and cancer therapy.
Creative Biogene is passionate about strong customer orientation and high-quality service. We continuously challenge ourselves to stay ahead and remain one of the leading mRNA delivery service providers worldwide. Want to get more information about our services? Please don't hesitate to contact us right now!
- Zhao, W., et al. (2018). "Lipid polymer hybrid nanomaterials for mRNA delivery." Cellular and molecular bioengineering, 11(5), 397-406.
- Siewert, C. D., et al. (2020). "Hybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA." Cells, 9(9), 2034.