Reporter Gene mRNA

The reporter gene is a foreign coding region connected with the promoter sequence or element in the expression vector. The expression vector is introduced into the cell to provide a means for measuring the promoter activity. Once expressed in cells, the reporter protein can be determined by directly measuring the reporter protein itself or evaluating its enzyme activity and correlating the strength of the fusion promoter element with the amount of the report protein produced. The ideal reporter gene is not endogenous expressed in the cells of interest, and is easy to conduct sensitive, quantitative, rapid, easy, accurate, repeatable and safe detection. Reporter gene analysis was initially developed as a powerful tool for studying traditional promoters and enhancers. Because of its ease of use, further report constructs are used to study the functions of other key sequence elements, including those that affect RNA stability, IRES elements, translation, and others. In conclusion, reporter genes and their related detection methods have made great contributions to the study of eukaryotic gene expression and regulation.

Creative Biogene provides pre-designed and stored reporter genes for tracking transfection efficiency and expression duration in cultured cells. These mRNA are similar to fully processed, capped, and polyadenylated mRNA and are ready for ribosome translation. These reporter gene mRNA were unmodified or modified with 5-methoxyuridine to reduce the innate immune response. Please browse our products to find what you're looking for.