Gene Gun Delivery of mRNA
Developing transfection methods for the manipulation of cell functions, especially for immune cells, such as macrophage and T cell, would be of greatest interest to both basic and applied translation studies. In this regard, different nucleic acid transfection strategies for cells have been investigated and several utilitarian techniques have been developed, such as electroporation (one of the oldest means of transfection), nucleofection, magnetofection, and gene gun. Among them, gene guns are utilized for some of the earliest mRNA therapeutic studies. Although compared with other transfection techniques using electroporation or lipids in culture cells, the transfection efficiency of gene gun would be lower, the technique has its particular advantage. Gene gun is always used to transfect cells that are unable to easily achieve by these methods, and for cells that are deep in tissues. Therefore, the method is applied for the delivery of nucleic acid into skin, muscle, in gene therapy, and among others. As an experienced supplier in biotech markets, Creative Biogene is devoted to offering customized mRNA transfection services for customers' particular needs in a high-level and cost-efficient manner. Aided by our well-established platforms and skillful technicians, we believe that our service will make your project achieve twice the result with half the effort.
Gene gun delivery of nucleic acid
Delivery with a gene gun, also called bio-ballistic or biolistic gene delivery, is a utilitarian technique for physical delivery of nucleic acid to target tissues, having the advantages of fast, simple, and flexible. The technique was first used to plant cell transformation for gene transformation and then adapted to mammalian cells a few years later. The principle of gene gun is to deliver nucleic acids by bombarding target cells with nucleic acid-coated microcarrier particles at high velocity. The target area of nucleic acid transfer can be focused and the surrounding tissue can be protected from bombardment within straightforward mechanical. The efficiency of the method depends on a series of factors, such as the size and density of microspheres, the material of carrier particles, the force of bombardment, kind of gene gun, and so on. These parameters markedly affect the competence of the gene transfer of gene gun and need to be adjusted according to the targeted cells and tissues. Previous studies had applied gene guns to many animals, including mice, rabbits, guinea pigs, and so on. The method is widely used for in vivo and in vitro transfection studies in animals and cell lines.
Fig1. Gene gun for DNA delivery to cells. (Jinturkar, K. A., et al., 2011)
The advantages of gene gun system over common needle injection system
- Facilitates direct transfer of the nucleic acid in various tissues, not affected by the kinds of cell, receptors, and molecules at the cell surface.
- The degradation of transfected nucleic acid is reduced.
- Allow delivery of several genes simultaneously to the same target tissues.
Service offering in Creative Biogene
Gene gun has particular advantages, such as facilitating overcoming barriers such as the cell wall. The method is simple, fast and flexible, and can efficiently transfect a small amount of genes for transcription results with less toxicity profile. Gene gun delivery of mRNA in situ is very efficient for transgene expression and genetic immunization. We offer a gene gun-based mRNA delivery service to help our customers to direct introduce mRNA in various biological systems like skin and muscles. In addition, we can also accept a series of cells for transfection, including NIH 3T3, macrophage cell line, mouse liver in situ, and others. Based on our well-established platforms and skillful technicians, we can offer an optimal gene gun-based cell transfer strategy and service tailored to specific applications.
Benefits of our service
- Higher local tissue transfection efficiency
- Transfection in various cell type
- Optimized process standardization
If you are interested in our services, please contact us for more information. We look forward to providing services for your next project.
- Jinturkar, K. A., et al. "Gene delivery using physical methods." Challenges in delivery of therapeutic genomics and proteomics. Elsevier, 2011. 83-126.
- O'Brien, J. A., & Lummis, S. C. (2011). "Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles." BMC biotechnology, 11(1), 1-6.