Microinjection Delivery of mRNA
Over the years, a wide range of methods have been developed for the introduction of mRNA into cells. Of these, microinjection is the most direct method and is currently used for multiple applications. At present, microinjection to single cells has been applied for transduction-challenged cells, production of transgenic animals, and conducting in vitro fertilization. Although microinjection has the disadvantage of tedious and limited (since it allows modus operandi with only one cell at a time), the method continues to be commonly used for gene delivery with mRNA to large cells, which will help researchers to study single cells for complicated cellular processes, structure, as well as functions in vitro. As a forward-looking research institute and an experienced supplier in biotech markets, Creative Biogene is devoted to offering customized mRNA transfection services based on a series of techniques, including microinjection. Our computer-assisted and microprocessor-controlled injection systems allow for quantitative microinjection with high injection rates and reproducible data.
Microinjection for cell transfection
Microinjection is a process that carries out biological material delivery via the insertion of a micropipette into living cells in culture. This technique can be applicable across a wide range of cell types, especially in vitro delivery to hard-to-transfect cells. Therefore, the microinjection system can function as a powerful tool and has been applied to many biomedical applications, including the direct introduction of nucleic acids into the cell cytoplasm or nucleus. Unlike conventional cell transfection, microinjection allows the researcher to effectively introduce nucleic acids into the cell within a very narrow timeframe. In addition, microinjection has unique advantages for single-cell transfection, such as cost-effectiveness, availability to different cell types, applicability to injection substances, higher security, and so on. With the development of technology, there are several microinjection systems that have been integrated with robotics technology, allowing automated injection with high transfection efficiency.
Fig1. Microinjection of DNA into the nucleus of a single cell. (Jinturkar, K. A., et al.,2011)
Benefits of microinjection in cell transfection
- Cost-effective, the amount of injected material delivered can be precious control.
- High efficiency of transduction.
- Wide applicability, available to different cell types and injection substances.
- Low cytotoxicity, by virtue of its virus-free nature.
Service offering in Creative Biogene
With our well-established platform, our quantitative microinjection service can be applied to study cell responses to different dosages of IVT mRNA. Our scientists are trained in microinjection technology and have established a systematic and standardized cell preparation. The automated micromanipulation and microinjection processes allow high injection rates with reproducible results, ensuring quantitative microinjection. We support the challenged primary cell transfection through precise microinjection, such as mesenchymal stem cells and post-mitotic neuron cells.
Specific services include
Benefits of mRNA injections
- No need to use RNA Polymerase inhibitors, which might affect cells in the regulation of the overall metabolism of RNA.
- mRNA can be modified in vitro prior to injection, which can help to investigate the effect of these features.
- The exact volumes of injected mRNA can be roughly estimated.
Simply let us know about your research needs. We will propose the best strategy to best match your research objectives. Contact us right now!
- Jinturkar, K. A., et al. (2011). "Gene delivery using physical methods." Challenges in delivery of therapeutic genomics and proteomics. Elsevier, 2011. 83-126.
- Chow, Y. T., et al. (2016). "Single cell transfection through precise microinjection with quantitatively controlled injection volumes." Scientific reports, 6(1), 1-9.
- Tolmachov, O. E., & Tolmachova, T. (2015). "Methods of transfection with messenger RNA gene vectors." Gene Therapy-Principles and Challenges, 1-55.