|These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNA contain a poly(A) tail at the 16’ end and are also optimized to yield improved stability and performance.
|Protein expression directly in cytoplasm without nuclear uptake; faster protein expression than DNA transfection; no genome integration; protein expression directly related to mRNA amount; ideal for transfecting slow or non-dividing cells; The protein is expressed by means of a promoter.
|β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyzes the conversion of β-galactosides into monosaccharides. It is a common marker gene used to assess transfection efficiency.