|These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNA contain a poly(A) tail at the 11’ end and are also optimized to yield improved stability and performance.
|Protein expression directly in cytoplasm without nuclear uptake; faster protein expression than DNA transfection; no genome integration; protein expression directly related to mRNA amount; ideal for transfecting slow or non-dividing cells; The protein is expressed by means of a promoter.
|F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.