|These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNA contain a poly(A) tail at the 6’ end and are also optimized to yield improved stability and performance.
|Protein expression directly in cytoplasm without nuclear uptake; faster protein expression than DNA transfection; no genome integration; protein expression directly related to mRNA amount; ideal for transfecting slow or non-dividing cells; The protein is expressed by means of a promoter.
|GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.