mRNA mCherry (5moU)
|Description||These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance. They are modified with 5-methoxyuridine (15moU replaces U) to reduce innate immune responses.|
|Benefits||No nuclear uptake is required - proteins are directly expressed in the cytoplasm; Faster protein expression than DNA transfection; No genome integration; It is very suitable for transfection of slow or non dividing cells; The protein is expressed in a manner independent of the total promoter.|
|Applications||mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching.|
For research use only. Not intended for any clinical use.