mRNA GFP (5moU)
|Description||These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance. They are modified with 5-methoxyuridine (10moU replaces U) to reduce innate immune responses.|
|Benefits||No nuclear uptake is required - proteins are directly expressed in the cytoplasm; Faster protein expression than DNA transfection; No genome integration; It is very suitable for transfection of slow or non dividing cells; The protein is expressed in a manner independent of the total promoter.|
|Applications||GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 511 nm.|
For research use only. Not intended for any clinical use.