Overview

RNA oligonucleotides are essential tools for studying gene regulation, developing RNA-based therapeutics, and enabling high-throughput screening. Our custom RNA synthesis service provides researchers with high-purity, sequence-specific RNA oligos, from standard short RNAs to longer modified constructs required for advanced applications.

We offer a flexible synthesis platform that accommodates a wide variety of chemical modifications, including 2′-O-methyl, phosphorothioate backbone, and locked nucleic acid (LNA) substitutions, as well as fluorescent labels and biotin tags. Each oligo is synthesized under strict quality control using HPLC or PAGE purification, and verified by mass spectrometry to ensure the highest purity and integrity.

Our service is designed to support the entire workflow of RNA-based research — from early discovery and lead optimization to preclinical development. Whether you require small interfering RNAs (siRNAs), microRNA mimics, short hairpin RNAs (shRNAs), or synthetic guide RNAs for CRISPR, we deliver custom RNA oligos that meet your precise specifications.

Key Capabilities and Customization Options

Explore Our RNA Synthesis Platform →

RNA Oligonucleotide Types and Modalities

We support a wide range of RNA modalities commonly used in research and therapeutic development. Each can be custom-synthesized with the modifications and purity required for your specific assay or application.

• Small Interfering RNA (siRNA): Short double-stranded RNA molecules (typically 19-23 bp) designed to induce RNA interference. We offer custom siRNA with a variety of chemical modifications to enhance stability, reduce off-target effects, and improve potency.
• MicroRNA (miRNA) Mimics and Inhibitors: Synthetic RNA molecules that mimic endogenous miRNAs or act as inhibitors (antagomirs) to modulate miRNA activity. Useful for studying gene regulation and therapeutic target validation.
• Short Hairpin RNA (shRNA): Single-stranded RNA that folds into a stem-loop structure for expression in cells. We synthesize shRNA sequences compatible with plasmid or viral vector delivery.
• Synthetic Guide RNA (sgRNA): Single guide RNA for CRISPR-Cas systems. We provide custom sgRNA with optional modifications to improve stability and editing efficiency, suitable for genome engineering applications.
• Long RNA and mRNA Constructs: Custom RNA oligonucleotides up to 100+ nucleotides, including modified mRNA for protein expression, long non-coding RNA, and antisense RNA. Our synthesis capabilities include chemical modifications to enhance translation and reduce immunogenicity.

Streamlined RNA Synthesis Workflow

From sequence submission to final delivery, our process is designed for efficiency and transparency. Each step is supported by rigorous quality checks to ensure you receive the highest quality RNA oligonucleotide for your research.

• Sequence Design and Review: Submit your desired RNA sequence through our online ordering system. Our experts review the sequence for potential issues (e.g., secondary structure, stability) and suggest modifications to improve performance.
• Solid-Phase Synthesis: RNA oligonucleotides are synthesized using standard phosphoramidite chemistry on controlled pore glass (CPG) supports. Modifications are incorporated during synthesis, and the RNA is cleaved and deprotected under controlled conditions.
• Purification and QC: Crude RNA is purified using HPLC or PAGE to achieve the desired purity level. Each batch is analyzed by UV spectrophotometry, mass spectrometry (ESI-MS or MALDI-TOF), and analytical HPLC to confirm identity and purity.
• Final Quality Release and Shipping: After passing all quality control criteria, the purified RNA is lyophilized and shipped with a Certificate of Analysis. We offer optional aliquoting and custom packaging to meet the needs of your downstream applications.

Timeline and Delivery

Turnaround times depend on sequence length, modifications, purification requirements, and scale. Our team will provide a specific timeline upon request. Standard orders are typically processed within 2–4 weeks after sequence review.

• Order Submission: Submit sequence and specifications via our online platform or direct contact.
• Sequence Review: Our team reviews the sequence and confirms feasibility within 1–2 business days.
• Synthesis and Purification: RNA is synthesized, cleaved, deprotected, and purified according to your chosen options.
• Quality Control: Full QC analysis including mass spectrometry, HPLC, and UV quantification.
• Shipment: Lyophilized RNA shipped with Certificate of Analysis, typically within 1–2 weeks after QC release.

Expedited options are available for qualified projects; contact our customer service team for details.

Quality Assurance and Control

Quality is the foundation of our custom RNA synthesis service. We implement rigorous QC procedures at every step, from raw material qualification to final product release.

Each RNA oligonucleotide is analyzed by mass spectrometry (ESI or MALDI-TOF) to confirm molecular weight and detect any truncation products. HPLC or PAGE analysis provides purity assessment, and UV quantification ensures accurate yield. All QC data is compiled into a Certificate of Analysis (CofA) that accompanies the shipment.

For modified RNA, additional QC measures such as nuclease stability assays or endotoxin testing can be arranged upon request. Our synthesis platform is operated under strict quality management systems to ensure batch-to-batch reproducibility and reliability.

Why Choose Our Custom RNA Synthesis Service?

• Broad Modification Capabilities: Access to a wide range of chemical modifications, including 2′-O-methyl, 2′-fluoro, phosphorothioate, LNA, and fluorescent labels, to tailor RNA stability, activity, and detection.
• Flexible Scales and Purification: Synthesis from 0.2 µmol to >100 µmol, with desalting, HPLC, or PAGE purification options to meet diverse purity and yield requirements.
• Expert Scientific Support: Our RNA chemists provide guidance on sequence design, modification selection, and application optimization, helping you avoid common pitfalls.
• Comprehensive Quality Documentation: Every order includes a Certificate of Analysis with mass spec, HPLC trace, and UV quantification, providing full traceability and confidence in your results.
• Fast and Reliable Turnaround: Standard orders are processed within 2–4 weeks, with expedited options available for time-sensitive projects. Contact our team for specific timelines.

Applications and RNA Product Types

Our custom RNA oligonucleotides are used across a broad spectrum of research areas, including gene regulation, therapeutic development, and functional genomics. Below are common RNA product types we synthesize.

• siRNA: Double-stranded RNA for RNA interference studies. We offer custom siRNA with modifications to reduce off-target effects and enhance serum stability.
• miRNA Mimics and Inhibitors: Synthetic RNA molecules that modulate microRNA activity, used in target validation and disease modeling.
• shRNA: Single-stranded RNA that forms a hairpin structure for sustained gene silencing in cells.
• sgRNA: Guide RNA for CRISPR-Cas9/12a genome editing. Customized with modifications to improve editing efficiency and specificity.
• mRNA and Long RNA: Modified mRNA for protein expression or long non-coding RNA studies. We support synthesis of sequences up to 100+ nucleotides.

Contact Us

Whether you are developing RNA therapeutics, evaluating gene silencing candidates, or optimizing CRISPR guide RNA designs, our scientific team is available to help you select the most appropriate synthesis scale, purification method, and chemical modifications based on your research objectives. Contact us today to learn more about our Custom RNA Oligonucleotide Synthesis service or discuss tailored solutions for your next project.