T4 RNA ligase 1

Cat.No. MRM-011

Description T4 RNA Ligase 1 is an ATP-dependent ligase, active on a broad range of substrates including single-strand RNA, DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The enzyme catalyzes the formation of a phosphodiester bond between a 5'-phosphoryl-terminated nucleic acid donor to a 3'-hydroxyl-terminated nucleic acid acceptor in a template-independent manner.
Concentration 10U/μL
Storage Conditions -20℃
Storage Buffer 10mM Tris-HCl pH 7.5, 25℃, 0.1mM EDTA, 500mM KCl, 1mM DTT, and 50% (v/v)Glycerol.
Source Recombinant E.coli
Purity >95% by SDS-PAGE
Quality Assurance Free of endonuclease, exonuclease and RNase activities.
For research use only. Not intended for any clinical use.
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