T4 RNA ligase 1
|Description||T4 RNA Ligase 1 is an ATP-dependent ligase, active on a broad range of substrates including single-strand RNA, DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The enzyme catalyzes the formation of a phosphodiester bond between a 5'-phosphoryl-terminated nucleic acid donor to a 3'-hydroxyl-terminated nucleic acid acceptor in a template-independent manner.|
|Storage Buffer||10mM Tris-HCl pH 7.5, 25℃, 0.1mM EDTA, 500mM KCl, 1mM DTT, and 50% (v/v)Glycerol.|
|Purity||＞95% by SDS-PAGE|
|Quality Assurance||Free of endonuclease, exonuclease and RNase activities.|
For research use only. Not intended for any clinical use.